17. SRS Microscope

Technique 

The method is based on Stimulated Raman Scattering (SRS) in which two beams, pump and Stokes are incident on the sample. When the difference in frequency pump - Stokes matches a molecular vibration, stimulated excitation of the vibrational transition occurs. This process causes an intensity loss in the pump beam) and an intensity gain in the Stokes beam. The change in pump or Stokes beams can be measured via lock-in detection.

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Units

Microscope, laser system, lock-in detection.            

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Instrument type

Homemade/Commercial

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Complexity level

High

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Applications

The setup provides background-free Raman imaging with improved image contrast, both of which are important for biomedical imaging applications.